Flow-through comb electroporation device for delivery of macromolecules.

Selective High Efficiency In-flow Electroporation with Focused Electric Fields in a Microsystem

We present a new technique for delivery of macromolecules into biological cells in suspension by in-flow electroporation which achieves reproducible high delivery efficiency with excellent post-electroporation viability and enables delivery to selected cell types in mixtures. We detect cells via impedance change as they flow through a microfabricated sense-porate aperture and then electroporate...

Focal macromolecule delivery in neuronal tissue using simultaneous pressure ejection and local electroporation

Electroporation creates transient pores in the plasma membrane to introduce macromolecules within a cell or cell population. Generally, electrical pulses are delivered between two electrodes separated from each other, making electroporation less likely to be localised. We have developed a new device combining local pressure ejection with local electroporation through a double-barrelled glass mi...

A Review on Current Status and Future Potential of Transdermal Patches as a Promising Drug Delivery System

Transdermal patches are now widely used in topical and transdermal drug delivery systems (TDDS). Up to now, TDDS has been used in several conditions, such as smoking cessation, analgesic effect, nausea, contraception, and hormone therapy. Basically, there are two types of transdermal patches: the reservoir-type patches and the matrix-type patches. First generation TDDS were designed for deliver...

Possibility of electroporation with microneedle-electrode array for transdermal delivery of macromolecules

A practical assessment of transdermal drug delivery by skin electroporation.

Transdermal drug delivery has many potential advantages, but the skin's poorly-permeable stratum corneum blocks delivery of most drugs at therapeutic levels. Short high-voltage pulses have been used to electroporate the skin's lipid bilayer barriers and thereby deliver compounds at rates increased by as much as four orders of magnitude. Evidence that the observed flux enhancement is due to phys...